Transcription profiling of human HCT116 clones stably expressing vegf 5 prime UTR reveals a novel tumor promoting function

ABSTRACT: VEGF165 is one of the key regulators of tumor development. Using HCT116 cells transfected with full-length vegf mRNA, full-length vegf mRNA with mutations of H9D and L14E, mutated vegf mRNAs lacking untranslated regions (UTRs), and 5â??UTR mutated between nt 591 and nt 746, we found that vegf 5â??UTR resided an anti-apoptotic activity against chemotherapy independently of VEGF165. We next established HCT116 clones stably expressing vegf 5â??UTR or the mutated vegf 5â??UTR. The cells expressing 5â??UTR, but not the mutated UTR, showed the drug-resistant phenotype, anchorage-independent growth, and rapid tumor growth when implanted in athymic nude mice. Microarray and real-time PCR showed that the vegf 5â??UTR-expressing tumors up-regulated anti-apoptotic genes including mia and down-regulat

ORGANISM(S): Homo sapiens

SUBMITTER: Tomoko Kawai 

PROVIDER: E-GEOD-8888 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress