Comparsion of Smad1 and Smad5 Dependent Transcript Profiles in Zebrafish

ABSTRACT: The BMP signaling pathway regulates multiple steps of hematopoiesis, mediated through receptor-regulated Smads, including Smad1 and Smad5. Here we use loss-of-function approaches in zebrafish to compare the roles of Smad1 and Smad5 during embryonic hematopoiesis. Microarray experiments revealed that the two proteins regulate redundantly the key initiators of the hemato-vascular program, including scl, lmo2, and gfi1. However, each also regulates a remarkably distinct genetic program, with Smad5 uniquely regulating the BMP signaling pathway itself. Our results suggest that specificity of BMP signaling output, with respect to hematopoiesis, can be explained by differential functions of Smad1 and Smad5. Keywords: Gene expression transcript profiles The experiment was designed to identify the

ORGANISM(S): Danio rerio

SUBMITTER: Lisa McReynolds 

PROVIDER: E-GEOD-8903 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress