Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of CHRF-288-11 and primary human megakaryocytic cell cultures provide novel insights into lineage-specific differentiation

ABSTRACT: This SuperSeries is composed of the following subset Series:

GSE3838: Temporal expression profile of CHRF-288 cell line after phorbol ester stimulation
CHRF-288 cells were cultured in the presence of 10 ng/mL phorbol ester (PMA) or equivalent volume of DMSO solvent (0.02%). Unstimulated control cells from time zero (exponentially growing CHRF cells) were also analyzed. For PMA treated legs, only the adherent cells were included in the transcriptional analysis. Two biological replicate experiments were analyzed and approximately one-half of the samples with each experiment were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference RNA pool labeled with Cy5.

GSE3839: Temporal expression profile of megakaryocytic differentiation primary CD34+ cell culture Experiment
G-CSF mobilized peripheral blood CD34-positive cells were cultured with TPO, IL-3, and Flt3-L to induce Mk differentiation. Samples prior to day 5, including uncultured starting cells, were analyzed directly, whereas samples after and including day 5 were positively selected for CD41a expression immediately prior to RNA isolation. Three biological replicate experiments were analyzed and approximately one-half of the samples from each experiment were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference RNA pool labeled with Cy5.

ORGANISM(S): Homo sapiens

SUBMITTER: Eleftherios Papoutsakis

PROVIDER: E-GEOD-8914 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Comparative, genome-scale transcriptional analysis of CHRF-288-11 and primary human megakaryocytic cell cultures provides novel insights into lineage-specific differentiation.

Fuhrken Peter G PG Chen Chi C Miller William M WM Papoutsakis Eleftherios T ET

Experimental hematology 20070301 3

<h4>Objectives</h4>Little is known about the transcriptional events underlying megakaryocytic (Mk) differentiation. We sought to identify genes and pathways previously unassociated with megakaryopoiesis and to evaluate the CHRF-288-11 (CHRF) megakaryoblastic cell line as a model system for investigating megakaryopoiesis.<h4>Methods</h4>Using DNA microarrays, Q-RT-PCR, and protein-level assays, we compared the dynamic gene expression pattern of phorbol ester-induced differentiation of CHRF cells ...[more]

PMID: 17309828

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Dataset Information

Transcription profiling of CHRF-288-11 and primary human megakaryocytic cell cultures provide novel insights into lineage-specific differentiation

Publications

Comparative, genome-scale transcriptional analysis of CHRF-288-11 and primary human megakaryocytic cell cultures provides novel insights into lineage-specific differentiation.

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