NF-?B Activation Controls Phagolysosome Fusion-Mediated killing of Mycobacteria by Macrophages

ABSTRACT: Mycobacterium smegmatis is a model non-pathogenic mycobacterium that is efficiently killed by macrophages. Here, we explore the role of NF-?B in the innate immune response, focusing in detail on the mechanisms of the first killing period (1-4h) of M. smegmatis which coincides with phagosome-lysosome fusion. We show that infection of macrophages with M. smegmatis induces an activation of NF-?B and this activation is required for killing since treatment of macrophages with NF-?B inhibitors or siRNA silencing of the NF-?B subunit p65 increases bacterial survival. NF-?B induced proteins were thus hypothesized to be essential during the first phase of M. smegmatis killing. We therefore identified, using RNA microarray, the genes that were regulated during infection in the absence and presence o

ORGANISM(S): Mus musculus

SUBMITTER: Bibhuti Mishra 

PROVIDER: E-GEOD-8999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress