T lymphocyte activation gene identification by coregulated expression on DNA microarrays

ABSTRACT: High-capacity methods for assessing gene function have become increasingly important because of the increasing number of newly identified genes emerging from large-scale genome sequencing and cDNA cloning efforts. We investigated the use of DNA microarrays to identify uncharacterized genes specifically involved in human T cell activation. Activation of human peripheral blood T lymphocytes induced significant changes in hundreds of transcripts, but most of these were not unique to T cell activation. Variation of experimental parameters and analysis techniques allowed better enrichment for gene expression changes unique to T cell activation. Best results were achieved by identification of genes that were most highly coregulated with the T-cell-specific transcript interleukin 2 (IL2) in a &qu

ORGANISM(S): Homo sapiens

SUBMITTER: Mao Mao 

PROVIDER: E-GEOD-902 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress