Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human umbilicial vein endothelial cells after TNF-alpha treatment

ABSTRACT: The many steps involved in the production of a mature mammalian mRNA are extensively coupled, and levels of both precursors and products can be measured using expression and genomic tiling microarrays. Different probes in these arrays targeting the same transcript often give different signals; then, precursor (nascent) RNA – which is present transiently at low concentrations – is difficult to detect. Experiment Overall Design: Using tumor necrosis factor a (TNF-alpha) to switch on transcription of selected human genes rapidly and synchronously, we apply samples collected every 15 min to arrays

ORGANISM(S): Homo sapiens

SUBMITTER: Shuichi Tsutsumi 

PROVIDER: E-GEOD-9055 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide studies reveal that transcription by RNA polymerase II (Pol II) is dynamically regulated. To obtain a comprehensive view of a single transcription cycle, we switched on transcription of five long human genes (>100 kbp) with tumor necrosis factor-alpha (TNFalpha) and monitored (using microarrays, RNA fluorescence in situ hybridization, and chromatin immunoprecipitation) the appearance of nascent RNA, changes in binding of Pol II and two insulators (the cohesin subunit RAD21 and the CC  ...[more]

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