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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Xenopus embryos

ABSTRACT: Xenopus laevis embryos were injected with mRNA for EFTFs at 2-cell stage. Animal caps collected at stage 9, cultured to the equivalent of stage 15 and RNA extracted. Four biological replicates of the EFTF-injected and GFP-injected (control) caps were used to profile transcript expression patterns using Affymetrix Xenopus Laevis GeneChip microarrays. Experiment Overall Design: Xenopus laevis embryos were injected with mRNA for EFTFs at 2-cell stage. Animal caps collected at stage 9, cultured to the equivalent of stage 15 and RNA extracted. Four biological replicates of the EFTF-injected and GFP-injected (control) caps were used to profile transcript expression patterns using Affymetrix Xenopus Laevis GeneChip microarrays.

ORGANISM(S): Xenopus laevis

SUBMITTER: Michael Zuber

PROVIDER: E-GEOD-9173 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Generation of functional eyes from pluripotent cells.

Viczian Andrea S AS Solessio Eduardo C EC Lyou Yung Y Zuber Michael E ME

PLoS biology 20090818 8

Pluripotent cells such as embryonic stem (ES) and induced pluripotent stem (iPS) cells are the starting point from which to generate organ specific cell types. For example, converting pluripotent cells to retinal cells could provide an opportunity to treat retinal injuries and degenerations. In this study, we used an in vivo strategy to determine if functional retinas could be generated from a defined population of pluripotent Xenopus laevis cells. Animal pole cells isolated from blastula stage ...[more]

PMID: 19688031

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