Genome-scale RNAi profiling of cell division in human tissue culture cells

ABSTRACT: Cell division is a fundamental process for multicellula organisation. Here we report a genome-scale RNAi screen in HeLa cells designed to identify human genes important for cell division. We utilized a library of endoribonuclease-prepared siRNAs (esiRNAs) for gene-silencing and used DNA content analysis to uncover genes that induced cell cycle arrest or altered ploidy upon knockdown. Rigorous validation and secondary assays were preformed to generate a nine-parameter fingerprint for each of the genes. These phenotypic signatures allowed the assignment of genes to specific functional classes by combining hierarchical clustering, cross-species analysis and proteomic data mining. We highlight the richness of our dataset by describing novel functions to genes in mitosis and cytokinesis.

ORGANISM(S): Homo sapiens

SUBMITTER: Anne-Kristine Heninger 

PROVIDER: E-GEOD-9176 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress