Transcription profiling of Saccharomyces cerevisiae for different levels of glucose

ABSTRACT: To characterize gene expression that is dependent on the strength of calorie restriction (CR), we obtained transcriptome at different levels of glucose, which is a major energy and carbon source for budding yeast. To faithfully mimic mammalian CR in yeast culture, we reconstituted and grew seeding yeast cells in fresh 2% YPD media before inoculating into 2%, 1%, 0.5% and 0.25% YPD media to reflect different CR strengths. We collected and characterized 160 genes that responded to CR strength based on the rigorous statistical analyses of multiple test corrected ANOVA (adjusted é value < 0.1 or raw é value < 0.0031) and Pearson correlation (|r| > 0.7). Based on the individual gene studies and the GO Term Finder analysis of 160 genes, we found that CR dosedependently and gradually increased

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Cheol-Koo Lee 

PROVIDER: E-GEOD-9217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress