Unknown,Transcriptomics,Genomics,Proteomics

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RNA quality and the influence on gene expression


ABSTRACT: We created RNA with different degree of degradation and investigated if the RNA quality influenced gene expression We used oligo-cDNA-microarrays to compare gene expressions Keywords: RNA quality, breast cancer, gene expression Six breast cancer samples were used, each sample were divided in four and the pieces were left in room temperature for four different time periods (50 sec, 2-3 min, 10 min, 30 min) Stratagene Universal Human RNA was used as reference

ORGANISM(S): Homo sapiens

SUBMITTER: Carina Strand 

PROVIDER: E-GEOD-9219 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RNA quality in frozen breast cancer samples and the influence on gene expression analysis--a comparison of three evaluation methods using microcapillary electrophoresis traces.

Strand Carina C   Enell Johan J   Hedenfalk Ingrid I   Fernö Mårten M  

BMC molecular biology 20070522


<h4>Background</h4>Assessing RNA quality is essential for gene expression analysis, as the inclusion of degraded samples may influence the interpretation of expression levels in relation to biological and/or clinical parameters. RNA quality can be analyzed by agarose gel electrophoresis, UV spectrophotometer, or microcapillary electrophoresis traces, and can furthermore be evaluated using different methods. No generally accepted recommendations exist for which technique or evaluation method is t  ...[more]

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