Transcription profiling of S. cerevisiae treated with absence of oxygen or depletion of ATP to investigate control of glycolytic enzyme fluxes

ABSTRACT: Metabolic fluxes may be regulated "hierarchically," e.g., by changes of gene expression that adjust enzyme capacities (V(max)) and/or "metabolically" by interactions of enzymes with substrates, products, or allosteric effectors. In the present study, a method is developed to dissect the hierarchical regulation into contributions by transcription, translation, protein degradation, and posttranslational modification. The method was applied to the regulation of fluxes through individual glycolytic enzymes when the yeast Saccharomyces cerevisiae was confronted with the absence of oxygen and the presence of benzoic acid depleting its ATP. Metabolic regulation largely contributed to the approximately 10-fold change in flux through the glycolytic enzymes. This contribution varied from 50 to 80%,

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jean-Marc Daran 

PROVIDER: E-GEOD-9232 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress