Transcription profiling of human polymorphonuclear leukocytes (PMNs) from healthy individuals vs patients with X-linked chronic granulomatous disease

ABSTRACT: Polymorphonuclear leukocytes (PMNs) were obtained from healthy individuals (Healthy1, Healthy2, Healthy3, and Healthy4) or patients with X-linked chronic granulomatous disease (XCGD1, XCGD2, XCGD3, XCGD4, XCGD5, and XCGD6). The studies were performed in accordance with a protocol approved by the Institutional Review Board for Human Subjects, National Institute of Allergy and Infectious Diseases, and the Institutional Review Board for Human Subjects at the University of Iowa. All studies were conducted according to Declaration of Helsinki principles. PMNs (107) were combined with or without IgG and C3bi-coated latex beads (8 x 107) in wells of a 12-well tissue culture plate (pre-coated with 20% normal human serum) and centrifuged at 350 x g for 8 min at 4°C to synchronize phagocytosis. For

ORGANISM(S): Homo sapiens

DISEASE(S): XCGD

SUBMITTER: Frank DeLeo 

PROVIDER: E-GEOD-935 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress