Transcription profiling of nouse pigmented epithial cells

ABSTRACT: Adult (6- to 8-week-old) C57BL/6 purchased from CLEA Japan Inc. (Tokyo, Japan) were used as donors ocular pigment epithelium (PE). To cultivate retinal PE (RPE) eyes were cut into two parts along a circumferential line posterior to the ciliary process, creating a ciliary body-deficient posterior eyecup. The eyecup was then incubated in 0.2% trypsin (Biowhitaker) for 1 hr. These tissues were triturated to make a single cell suspension, and then re-suspended in the medium. Experiment Overall Design: Comparison of gene expression pattern in Mouse Primary Cultured Cell Lines (RPE, RPE+IFNg).

ORGANISM(S): Mus musculus

SUBMITTER: Yuri Futagami 

PROVIDER: E-GEOD-9428 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress