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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Saccharomyces cerevisiae nmd2::HIS3 GAL-NMD2 strain

ABSTRACT: The goal of this set of experiments was to identify transcripts that are differentially expressed upon reactivation of NMD in an nmd2::HIS3 strain by galactose-induced expression of the NMD2 gene. Experiment Overall Design: Yeast strains harboring nmd2::HIS3 and the GAL1-NMD2 allele on a plasmid or the empty vector were grown in SC-medium containing raffinose but lacking uracile. Genome-wide expression profiles of the GAL-NMD2 and nmd2 deletion strains were analyzed over a 60-minute time course after adding galactose to the cell cultures. Four independent replicates were carried out for this experiment.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Feng He

PROVIDER: E-GEOD-9482 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Association of yeast Upf1p with direct substrates of the NMD pathway.

Johansson Marcus J O MJ He Feng F Spatrick Phyllis P Li Chunfang C Jacobson Allan A

Proceedings of the National Academy of Sciences of the United States of America 20071217 52

Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that detects and degrades transcripts containing premature translation termination codons. Gene expression profiling experiments have shown that inactivation of the NMD pathway leads to the accumulation of both aberrant, nonsense-containing mRNAs, and many apparently wild-type transcripts. Such increases in transcript steady-state levels could arise from direct changes in the respective mRNA half-lives, or indirectly, as a consequenc ...[more]

PMID: 18087042

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GAL-NMD2

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