A Transcriptome Database for Astrocytes, Neurons, and Oligodendrocytes

ABSTRACT: A Transcriptome Database for Astrocytes, Neurons, and Oligodendrocytes: A New Resource for Understanding Brain Development and Function Understanding the cell-cell interactions that control CNS development and function has long been limited by the lack of methods to cleanly separate astrocytes, neurons, and oligodendrocytes. Here we describe the first method for the isolation and purification of developing and mature astrocytes from mouse forebrain. This method takes advantage of the expression of S100β by astrocytes. We used fluorescent activated cell sorting (FACS) to isolate EGFP positive cells from transgenic mice that express EGFP under the control of an S100β promoter. By depletion of astrocytes and oligodendrocytes we obtained purified populations of neurons, while by panning w

ORGANISM(S): Mus musculus

SUBMITTER: John Cahoy 

PROVIDER: E-GEOD-9566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress