Transcription profiling of S. cerevisiae GPCR mutants gpr1 and gpa2

ABSTRACT: Experiment design,Type of experiment: Gene expression profiling analysis of S.cerevisiae W303-1a.,Experimental factor: genetic variation (WT, gpr1 and gpa2 mutant strains).,Number of hybridizations: 6. Single-colour labeling was performed for each sample.,Sample preparation:,Total RNA was prepared using FastRNA Pro Red Kit (BIO 101 Systems) according to the manufacturers' instructions.,Labeling protocol,10 µg of total RNA was reverse transcribed using the SuperScript Choice system for cDNA synthesis (Life Technologies) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5’-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3’ (Genset Oligo). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifu

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Katsuhiko Shirahige 

PROVIDER: E-GEOD-970 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress