Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Brassica napus flowers of two isogenic lines J10AB and J03AB at different developmental stages

ABSTRACT: Dominant genic male sterility (DGMS) is a valuable system for hybrid seed production and improvement of plant populations, but the molecular mechanism of DGMS is not well understood. To facilitate the analysis of mechanism underlying DGMS, we compared gene expression profiles between fertile and sterile flowers of two pair of Brassica napus isogenic lines: J10AB and J03AB which only differ in sterility-related locus and fertility restore-related locus respectively by using Arabidopsis thaliana ATH-1 microarray. Gene expression profiling revealed that 360 genes differed in expression between the two lines and the number of differently expressed genes increased with progressing developmental stages. Cluster analysis result indicates that most important gene expression changes occur from PMC (anther less than 1mm) to uninucleate stage (anther in 3mm). There are 182 differential expressed genes detected between fertile plants and sterile plants at same stage. Some important pathways related to fertility control by KEGG analysis, such as glycolysis/gluconeogenesis pathway, flavonoid biosynthesis pathway and fatty acid metabolism pathway. Another abortion usually associated with suppression of gene expression of these pathways. Experiment Overall Design: The Homozygous DGMS two-type line J10AB was compared with heterozygous two-type line J03AB at three different stages of development: tetrad stage, uninucleate stage and trinucleate stage (Flower buds <1, 1-3 and >3mm, respectively) which were confirmed by cytology observation. All these flower buds were collected by hand. For each stage, two biological repetitions and two experimental repetitions were used, resulting in four hybridizations per developmental stage. Gene expression was compared between lines at different stages of development, as well as between developmental stages of fertile plants and sterile plants of each line.

ORGANISM(S): Brassica napus

SUBMITTER: hua cai dong

PROVIDER: E-GEOD-9721 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:In Arabidopsis, jasmonate is required for stamen and pollen maturation. Mutants deficient in jasmonate synthesis, such as opr3, are male-sterile but become fertile when jasmonate is applied to developing flower buds. We have used ATH1 oligonucleotide arrays to follow gene expression in opr3 stamens for 22 hours following jasmonate treatment. In these experiments, a total of 821 genes were specifically induced by jasmonate and 480 repressed. Comparisons with data from previous studies indicate that these genes constitute a stamen-specific jasmonate transcriptome, with a large proportion (70%) of the genes expressed in the sporophytic tissue but not in the pollen. Bioinformatics tools allowed us to associate many of the induced genes with metabolic pathways that are likely up-regulated during jasmonate-induced maturation. Our pathway analysis led to the identification of specific genes within larger families of homologues that apparently encode stamen-specific isozymes. Extensive additional analysis of our dataset identified 13 transcription factors that may be key regulators of the stamen maturation processes triggered by jasmonate. Two of these transcription factors, MYB21 and MYB24, are the only members of subgroup 19 of the R2R3 family of MYB proteins. A myb21 mutant obtained by reverse genetics exhibited shorter anther filaments, delayed anther dehiscence and greatly reduced male fertility. A myb24 mutant was phenotypically wild type, but production of a myb21Â myb24 double mutant indicated that introduction of the myb24 mutation exacerbated all three aspects of the myb21 phenotype. Exogenous jasmonate could not restore fertility to myb21 or myb21Â myb24 mutant plants. Together with the data from transcriptional profiling, these results indicate that MYB21 and MYB24 are induced by jasmonate and mediate important aspects of the jasmonate response during stamen development. Experiment Overall Design: Three replicates for opr3 at each time point of 0 hour, 30 minutes, 2 hours, 8 hours and 22 hourss of either JA or OPDA treatment. One replicate of wild-type stamens. <br><br>Note that data files GSM106833.txt and GSM106907.txt as downloaded from GEO are identical.

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Transcription profiling of Brassica napus flowers of two isogenic lines J10AB and J03AB at different developmental stages

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