Transcription profiling of Escherichia coli tannin resistance strategies in anaerobic conditions

ABSTRACT: Gene expression profiles of Escherichia coli, grown anaerobically, with or without Acacia mearnsii (Black wattle) extract were compared to identify tannin-resistance strategies. The cell envelope stress protein, spy, and the multidrug transporter-encoding mdtABCD, both under the control of the BaeSR two-component regulatory system, were significantly up-regulated in the presence of tannins. BaeSR mutants were more tannin-sensitive than their wild-type counterparts. Experiment Overall Design: E. coli BW13711 cells were grown anaerobically in continuous culture (d = 0.14h-1) in the presence and absence of Acacia mearnsii (Black Wattle) tannin extract (WTE) at 37°C. Under both steady state conditions, E. coli reached an OD600 of ~0.2. The pH at steady-state was 5.7 and 5.3 without and with

ORGANISM(S): Escherichia coli

SUBMITTER: Alexandra Smith 

PROVIDER: E-GEOD-9755 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress