Transcription profiling of rice plants irradiated with carbon ion beams (HIMAC)

ABSTRACT: In this study, carbon-ion beams generated via the heavy-ion medical accelerator in Chiba (HIMAC) were targeted to growing rice seedlings (7-days-old) to examine their effect on this genome model. Both physiological parameters, such as growth, and molecular events, specially the gene expression profiles were examined immediately after irradiation (at 270 Gy dose) by rice oligo (22K) DNA microarrays. Genome-wide transcriptional profiling of radiation-response genes in rice provides us with first report on how our radioactive environment affects a plant species. Experiment Overall Design: An in vivo 7-days-old rice seedling model system was used, where whole seedlings were used for exposure to the emitted radiation from HIMAC (carbon ion beams). Dye-swap or reverse labeling with Cy3 and Cy5 d

ORGANISM(S): Oryza sativa

SUBMITTER: Randeep Rakwal 

PROVIDER: E-GEOD-9765 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress