Delayed cell cycling in DS Ts1Cje neural precursor cells results in gene expression dysregulation

ABSTRACT: Down syndrome neurophenotypes are characterized by mental retardation and a decreased brain volume. In order to identify whether deficits in proliferation, differentiation or survival could be responsible for this phenotype, neural precursor cells (NPCs) were isolated from the developing E14 neocortex of Down syndrome partial trisomy Ts1Cje mice and euploid (WT) littermates. Proliferation, cell differentiation and cell death assays revealed that Ts1Cje NPCs proliferated at a slower rate, due to a longer cell cycle and that a greater number of cells were positive for glial fibrillary acidic protein. An increase in Ts1Cje NPC cell death was also noted. Gene expression profiling was conducted on RNA extracted from Ts1Cje and WT NPCs. Approximately 54% of triploid gene expression ratios were s

ORGANISM(S): Mus musculus

SUBMITTER: Randal Moldrich 

PROVIDER: E-GEOD-9805 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress