Transcription profiling of mouse intestine in ENU mutagenesis mouse strains with missense mutations in Muc2 mucin and ER stress

ABSTRACT: Background; MUC2 mucin produced by intestinal goblet cells is the major component of the intestinal mucus barrier. MUC2 homo-oligomerizes intracellularly into large secreted polymers which give mucus its viscous properties. The inflammatory bowel disease (IBD) ulcerative colitis is characterized by depleted goblet cells and a reduced mucus layer, whereas goblet cells and the mucus layer are increased in the other major inflammatory bowel disease, Crohn’s disease. Methods and Findings; By murine N-ethyl-N-nitrosourea-mutagenesis we identified two distinct non-complementing missense mutations in Muc2 exons encoding N- and C-terminal homo-oligomerization domains causing an ulcerative colitis-like phenotype. Both strains developed mild spontaneous distal intestinal inflammation, chronic

ORGANISM(S): Mus musculus

SUBMITTER: Gareth Price 

PROVIDER: E-GEOD-9913 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress