Transcription profiling of human intra-pulmonary airways and buccal mucosa to identify the effects of cigarette smoke on the human airway epithelial cell transcriptome

ABSTRACT: A number of studies have shown that cigarette smoking produces a field defect, such that genetic mutations induced by smoking occur throughout the lung and its intra and extra-pulmonary airways. Based on this concept, we have begun this study, which has as its goal the definition of the normal airway transcriptome, an analysis of how that transcriptome is affected by cigarette smoke, and to explore the reversibility of altered gene expression when smoking has been discontinued. We have obtained brushings from intra-pulmonary airways (the right upper lobe carina) and scrapings from the buccal mucosa, from normal smoking and non-smoking volunteers (including 34 Current Smokers, 23 Never Smokers and 18 Former Smokers). RNA was isolated from these samples and gene expression profiles from intr

ORGANISM(S): Homo sapiens

SUBMITTER: Avrum Spira 

PROVIDER: E-GEOD-994 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress