Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional analysis of Leishmania infantum methotrexate resistant strains using full-genome DNA microarrays

ABSTRACT: Transcriptional analysis of the drug resistant strain L. infantum MTX20.5 compared to the wild-type strain The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L. infantum Keywords: drug resistance Two-condition experiment, resistant strain MTX20.5 vs wild-type. Four biological replicates for each strain, independently grown and harvested. One replicate per array

ORGANISM(S): Leishmania infantum

SUBMITTER: J Ubeda

PROVIDER: E-GEOD-9947 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy.

Ubeda Jean-Michel JM Légaré Danielle D Raymond Frédéric F Ouameur Amin Ahmed AA Boisvert Sébastien S Rigault Philippe P Corbeil Jacques J Tremblay Michel J MJ Olivier Martin M Papadopoulou Barbara B Ouellette Marc M

Genome biology 20080718 7

<h4>Background</h4>Drug resistance can be complex, and several mutations responsible for it can co-exist in a resistant cell. Transcriptional profiling is ideally suited for studying complex resistance genotypes and has the potential to lead to novel discoveries. We generated full genome 70-mer oligonucleotide microarrays for all protein coding genes of the human protozoan parasites Leishmania major and Leishmania infantum. These arrays were used to monitor gene expression in methotrexate resist ...[more]

PMID: 18638379

Similar Datasets

Methotrexate resistant Leishmania strains

Project description:This SuperSeries is composed of the following subset Series: GSE9947: Transcriptional analysis of Leishmania infantum methotrexate resistant strains using full-genome DNA microarrays GSE9948: Transcriptional analysis of Leishmania major methotrexate resistant strains using full-genome DNA microarrays Keywords: SuperSeries Refer to individual Series

2010-05-25 | E-GEOD-9949 | biostudies-arrayexpress

Transcriptional analysis of Leishmania major methotrexate resistant strains using full-genome DNA microarrays

Project description:Transcriptional analysis of the drug resistant strain L. major MTX60.4 compared to the wild-type strain LV39 The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.major LV39 Keywords: drug resistance Two-condition experiment, resistant strain MTX60.4 vs wild-type LV39. Four biological replicates for each strain, independently grown and harvested. One replicate per array

2010-05-25 | E-GEOD-9948 | biostudies-arrayexpress

Whole-genome comparative RNA expression profiling of axenic and intracellular amastigote forms of Leishmania infantum

Project description:Transcriptional analysis of L. infantum promastigote compared to L. infantum intracellular amastigote and transcriptional analysis of L. infantum promastigote compared to L. infantum axenic amastigote. The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.infantum. Keywords: stage and culture condition Intracellular amastigote analysis: Two-condition experiment, promastigote stage vs intracellular amastigote stage. Six biological replicates for each stage, independently grown and harvested. One replicate per array Axenic amastigote analysis: Two-condition experiment, promastigote stage vs axenic amastigote stage. Four biological replicates for each stage, independently grown and harvested. One replicate per array.

2010-05-14 | E-GEOD-13983 | biostudies-arrayexpress

Transcriptional analysis of Leishmania infantum antimony resistant strains using full-genome DNA microarrays

Project description:The aim of the experiment is to detect any gene expression modulation in an antimony resistant L.infantum Sb2000.1 strain compared to an antimony sensitive L.infantum WT strain. We want to identify the modulated genes associated with an antimony resistant phenotype. The Sb2000.1 resistant strain used is about 1000 times more resistant to SbIII than the WT sensitive strain. The full-genome DNA microarrays includes one 70-oligonucleotide probe for each gene of L. infantum. Keywords: drug resistance Two-condition experiment, resistant strain Sb2000.1 vs wild-type. Five biological replicates for each strain, independently grown and harvested. One replicate per array

2009-08-15 | E-GEOD-16965 | biostudies-arrayexpress

Transcriptional analysis of wild-type Leishmania species between both stages using full-genome DNA microarrays

Project description:Transcriptional analyses of L. infantum promastigote compared to L. infantum intracellular amastigote, and L. major promastigote compared to L. major intracellular amastigote The full-genome DNA microarray includes one 70mer-oligonucleotide probe for each gene of L. infantum and for each gene of L.major LV39 Keywords: stage-specific comparison Leishmania infantum: Two-condition experiment, promastigote stage vs amastigote stage. Six biological replicates for each stage, independently grown and harvested. One replicate per array Leishmania major: Two-condition experiment, promastigote stage vs amastigote stage. Four biological replicates for each stage, independently grown and harvested. One replicate per array

2008-05-28 | E-GEOD-10407 | biostudies-arrayexpress

Comparative Genomic Hybridization of Leishmania major methotrexate resistant strains vs wild-type LV39

Project description:CGH analysis of the drug resistant strain L. major MTX60.4 vs the wild-type strain LV39 The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.major LV39 Keywords: drug resistance Two-condition experiment, resistant strain MTX60.4 vs wild-type LV39. Three biological replicates for each strain, independently grown and harvested. One replicate per array

2008-05-31 | E-GEOD-11623 | biostudies-arrayexpress

KO comR vs. KO comX comparison

Project description:Transcriptional profiling of mutant Streptococcus thermophilus LMD-9 comR::lox72 (strain LF148) compared to mutant S. thermophilus LMD-9 DcomX (strain CB003) for the identification of the ComR regulon. Cells were grown in CDM medium supplemented with lactose 1% (CDML) and sampled at OD600 = 0.3~0.4 for mRNA extraction. 2 LF148 biological replicates (one used as a technical replicate for a dye swap), 2 CB003 biological replicates (one used as a technical replicate for a dye swap).

2014-02-15 | E-GEOD-35849 | biostudies-arrayexpress

Evidence of the role of tick subolesin in gene expression

Project description:Subolesin is an evolutionary conserved protein that was recently discovered in Ixodes scapularis as a tick protective antigen and has a role in tick blood digestion, reproduction and development. In other organisms, subolesin orthologs may be involved in the control of developmental processes. Because of the profound effect of subolesin knockdown in ticks and other organisms, we hypothesized that subolesin has a role in gene expression, thus affecting multiple cellular processes. The objective of this study was to provide support for the role of subolesin in gene expression. Keywords: time course Total RNA was prepared and pooled from subolesin dsRNA- and saline-injected ticks at 6 and 9 dpi (5 and 8 days of feeding).

2010-05-26 | E-GEOD-10222 | biostudies-arrayexpress

Runx1 (Aml1) knockdown in AMkL Meg-01 cells

Project description:Transcriptional profiling of the effect of shRNA silencing of Runx1 in human AMkL Meg-01 cells. RNA samples obtained from two independent colonies were compared to RNAs from negative control transductions in a two-color design. A total of four microarrays were completed, with a dye-swapped pair performed for each colony. Two-condition experiment, Runx1 knockdown vs. negative transduction controls. Biological replicates: 2 knockdown replicates, 2 control replicates.

2009-08-01 | E-GEOD-17311 | biostudies-arrayexpress

Trichoderma reesei QM6a CGH self hybridization

Project description:We perform a self hybridisation comprative genomic hybridization (CGH) in order to validate the probe tiling design we done on Trichoderma reesei. This hybridization was done using QM6a wild type strain. One biological replicate

2010-06-23 | E-GEOD-17752 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data