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Single cell sequencing of developing heart valves identifies novel cell populations and uncovers interstitial cell heterogeneity

ABSTRACT: This study has two main goals. The first is to define a gene expression atlas of heart valve cells and identify cell heterogeneity within postnatal heart valve development. The second is to identify interstitial cell populations involved in ECM remodeling during postnatal heart valve development. Overall design: Using droplet RNA sequencing, transcriptomes of single cells from P7 and P30 murine aortic and mitral heart valves were analyzed.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Alexia Hulin

PROVIDER: E-HCAD-18 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Maturation of heart valve cell populations during postnatal remodeling.

Hulin Alexia A Hortells Luis L Gomez-Stallons M Victoria MV O'Donnell Anna A Chetal Kashish K Adam Mike M Lancellotti Patrizio P Oury Cecile C Potter S Steven SS Salomonis Nathan N Yutzey Katherine E KE

Development (Cambridge, England) 20190312 12

Heart valve cells mediate extracellular matrix (ECM) remodeling during postnatal valve leaflet stratification, but phenotypic and transcriptional diversity of valve cells in development is largely unknown. Single cell analysis of mouse heart valve cells was used to evaluate cell heterogeneity during postnatal ECM remodeling and leaflet morphogenesis. The transcriptomic analysis of single cells from postnatal day (P)7 and P30 murine aortic (AoV) and mitral (MV) heart valves uncovered distinct sub ...[more]

PMID: 30796046

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Single cell sequencing of developing heart valves identifies novel cell populations and uncovers interstitial cell heterogeneity

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SOX9 modulates the expression of key transcription factors required for heart valve development

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SOX9 modulates the expression of key transcription factors required for heart valve development

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