Transcription profiling of mouse embryonic fibroblasts Rb-/- MEF (ME3) and Rb+/+ MEF (MEFA) during adipocyte differentiation
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ABSTRACT: Gene expression of Retinoblastoma deficient mouse embryonic fibroblasts (Rb-/- MEF, ME3) and wildtype mouse embryonic fibroblasts (Rb+/+ MEF,MEFA) during adipocyte differentiation were studied by cDNA microarray analysis. 2-day postconfluent cells (day 0) were treated with a hormonal cocktail including Rosiglitzone for differentiation into adipocytes. Gene expression at day 1, day 3, and day 8 after hormonal induction were compared to that before induction (day 0).
Project description:Gene expression timeseries analysis of mouse embryonic fibroblasts (MEF) during differentiation to mature adipocytes with a 27.648 element murine cDNA microarray. Three independent time series experiments of MEF adipocyte differentiation were performed in reference design. RNA from 7 time points (0d, 1d, 2d, 3d, 4d, 6d, 10d) was hybridized against RNA from the preconfluent stage to study the gene expression profile over the whole differentiation process.
Project description:Asbestos disease model: Occupational lung disease comprises a wide variety of disorders caused by the inhalation or ingestion of dust particles or noxious chemical. These disorders include pneumoconiosis, asbestos-related pleural and others. Although asbestos is carcinogenic to humans, the mechanism by which it induces cancer is unknown. The study from Brody AR has shown that lung fibroblasts are activated to proliferate and produce connective tissue during the development of lung fibrosis. The 129 mouse strain does not develop asbestos-induced fibrogenesis, whereas several other inbred strains rapidly respond to inhaled fibers. David Schwartz from Duke University has bred C57B6 (high responders) to 129 (low responder) and backcrossed the F1 mice to either C57B or 129. RNA has been collected from lungs of both parental and progeny mice and we are useing microarray to generate the gene express profile. The purpose for this study is to find out the genes (either up-regulated or down-regulated) involve in the inhalation of asbestos and provide a guideline to the further study of human inhalation disease.
Project description:Examination of gene expression associated with hypoxia treatment of parental salt sensitive (SS) and consomic Brown Norway (SS9BN) rat strain. SS9BN rats are derived from introgression of chromosome 9 from BN rats into the SS genetic background.
Project description:Examination of gene expression associated with hypoxia treatment of parental salt sensitive (SS) and consomic Brown Norway (SS9BN) rat strain. SS9BN rats are derived from introgression of chromosome 9 from BN rats into the SS genetic background.
Project description:For each strain, Ova-alum and alum-only sensitization is followed by ovalbumin challenge. Reactivity measured at 24, 48, 72 hrs for each group is compared to an ova-pre-control sample.
Project description:For each strain, Ova-alum and alum-only sensitization is followed by ovalbumin challenge. Reactivity measured at 24, 48, 72 hrs for each group is compared to an ova-pre-control sample.
Project description:For each strain, Ova-alum and alum-only sensitization is followed by ovalbumin challenge. Reactivity measured at 24, 48, 72 hrs for each group is compared to an ova-pre-control sample.