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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of E. coli HMS174(DE3)(pET30aNproGFPmut3.1) to analyze cellular response to full induction with IPTG

ABSTRACT: This transcription profiling time course experiment was conducted in order to analyze the cellular response of the T7 RNA polymerase based E. coli expression system HMS174(DE3)(pET30aNproGFP) to a single pulse induction with isopropyl-beta-D-galactoside (IPTG). The used cell material was produced under well controlled and defined conditions in an exponential carbon limited fed-batch cultivation at a growth rate of 0.1 per hour. The applied amount of 20µmol IPTG per gram cell dry weight assured a fully induced system and high recombinant gene expression rates. Non induced cells sampled at the time point of induction were used as reference and compared to cells subsequently sampled during the production period (sampling frequency 1 hour)

ORGANISM(S): Escherichia coli

SUBMITTER: Gerald Striedner

PROVIDER: E-MARS-16 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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