Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of E. coli BL21(DE3) to analyze the cellular response to high level expression of recombinant human super-oxide-dismutase (SOD)

ABSTRACT: This transcription profiling time course experiment was conducted in order to analyze the cellular response of E. coli BL21(DE3) to high level expression of recombinant human super-oxide-dismutase (SOD) on the transcriptional level. Three biological replicates were generated by using a carbon limited exponential fed-batch cultivation similar to industrial setups for large scale production. For induction of the system a single pulse of isopropyl-beta-D-galactoside (IPTG) yielding in a fully induced system is applied one doubling past feed start. In order to achieve a proper timeresolution of cellular responses sampling starts with a high frequency for the first hours past induction and decreases in the course of the experiment.

ORGANISM(S): Escherichia coli

SUBMITTER: Gerald Striedner 

PROVIDER: E-MARS-19 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mixtures of regression models for time course gene expression data: evaluation of initialization and random effects.

Scharl Theresa T   Grü Bettinan B   Leisch Friedrich F  

Bioinformatics (Oxford, England) 20091229 3

<h4>Summary</h4>Finite mixture models are routinely applied to time course microarray data. Due to the complexity and size of this type of data, the choice of good starting values plays an important role. So far initialization strategies have only been investigated for data from a mixture of multivariate normal distributions. In this work several initialization procedures are evaluated for mixtures of regression models with and without random effects in an extensive simulation study on different  ...[more]

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