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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of in vitro / in vivo osteoblast comparison-Comparison of in vivo and in vitro developed human osteoblasts

ABSTRACT: Comparison of the genetic profile of hBM-MSC differentiated in vitro into osteoblasts with in vivo developed osteoblasts derived from hip bone.

ORGANISM(S): Homo sapiens

SUBMITTER: paar Christine Paar

PROVIDER: E-MARS-4 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiation.

Kulterer Birgit B Friedl Gerald G Jandrositz Anita A Sanchez-Cabo Fatima F Prokesch Andreas A Paar Christine C Scheideler Marcel M Windhager Reinhard R Preisegger Karl-Heinz KH Trajanoski Zlatko Z

BMC genomics 20070312

<h4>Background</h4>Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influ ...[more]

PMID: 17352823

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Transcription profiling of human BM-MSC expanded from p2 until p10 and monitored by FACS, proliferation kinetics and differentiation ability

Project description:hBM-MSC were expanded from p2 until p10 and monitored by FACS, proliferation kinetics and differentiation ability;ÃÂµA analysis were performed p2/p5 (n=10)and p5/p10 (n=5) to examine if the genetic background of hBM-MSC is changeing during their high-grade expansion;

2007-06-09 | E-MARS-6 | biostudies-arrayexpress

Transcription profiling of human BM-MSC expanded from p2 until p10 and monitored by FACS, proliferation kinetics and differentiation ability

2007-06-09 | E-MARS-5 | biostudies-arrayexpress

Transcription profiling of mouse white adipose tissue and brown adipose tissue from Bscl2 knock-out mice and their wild-type control mice

Project description:Experiments were designed to compare white adipose tissue (WAT) or brown adipose tissue (BAT) -in male and female mice- between Bscl2 knock-out mice and their wild-type control mice. RNA was pooled to obtain 2x 8µg per tissue source and subjected to dye-swap hybridization.

2010-06-09 | E-MARS-18 | biostudies-arrayexpress

Transcription profiling of dfferentiation of human bone marrow derived hMSC with beta-glycerphosphate, ascorbicacidphosphate and dexamethasone for 21 days;evaluated time points d4, d7, d14 and d21 after induction

Project description:Differentiation of bone marrow derived hMSC with beta-glycerphosphate, ascorbicacidphosphate and dexamethasone for 21 days;evaluated time points d4, d7, d14 and d21 after induction; reference d-1 (one day before induction; preconfluent 70-80%)

2007-06-09 | E-MARS-3 | biostudies-arrayexpress

Transcription profiling of wild type and A-C/EBP transgenic mouse embryonic fibroblasts during adipogenesis to identify C/ebp alpha target genes

Project description:This experiment was designed to identify C/ebp alpha target genes during the process of adipogenesis by means of comparison of differentiating (wild-type) cells to a perturbation model (transgenic cells). For this, gene expression profiling during adipogenesis of mouse embryonic fibroblasts (MEFs) prepared from wild-type and transgenic mice was performed. The transgene encodes for a dominant-negative A-CEBP protein that abolishes C/EBP DNA-binding activity. Five timepoints were measured for both transgenic and wild-type MEFs during adipogenesis (0h, 10h, 20h, d3 , d8). Every timepoint was hybridized against pre-confluent cells as reference.

2009-07-02 | E-MARS-11 | biostudies-arrayexpress

Transcription profiling of human hMADS cells during adipocyte and osteoblast differentiation

Project description:human adipocyte and osteoblast differentiation of hMADS cells

2008-08-08 | E-MARS-10 | biostudies-arrayexpress

Transcription profiling of baboon organs to identify late host responses to Gram positive (S. pyogenes) or Gram negative (E. coli) induced sepsis

Project description:The aim of the project is to identify the late (24h) host response related to Gram positive (S. pyogenes) or Gram negative (E. coli) induced sepsis in a controlled sepsis model 24 hours a. Therefore a well defined baboon sepsis model, established at the Ludwig Boltzmann Institute for experimental and clinical traumatology of Vienna, is used. The overall goal of the project is to define differentially expressed or processed genes which give rise to diagnostic and therapeutic targets and consequently better monitoring and earlier onset of therapy.

2010-04-27 | E-MARS-15 | biostudies-arrayexpress

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2010-04-27 | E-MARS-14 | biostudies-arrayexpress

Transcription profiling of E. coli HMS174(DE3)(pET30aNproGFPmut3.1) to analyze cellular response to limited induction with IPTG

Project description:This transcription profiling time course experiment was conducted in order to analyze the cellular response of the T7 RNA polymerase based E. coli expression system HMS174(DE3)(pET30aNproGFP) to a limited induction with isopropyl-beta-D-galactoside (IPTG). The used cell material was produced under well controlled and defined conditions in an exponential carbon limited fed-batch cultivation at a growth rate of 0.1 per hour. One doubling past feed start induction was started by continuously feeding limiting amounts of inducer gaining in a constant IPTG to cell dry weight ratio of 1µmol/g. Due to the low induction level a physiologically tolerable recombinant gene expression rate was generated and production period was significantly elongated. Non induced cells sampled at the time point of induction were used as reference and compared to cells subsequently sampled during the production period (sampling frequency 2 hours).

2010-03-26 | E-MARS-17 | biostudies-arrayexpress

Transcription profiling by array of hMADS cells transfected with miR-26a and induced to differentiate into adipocytes

Project description:Human multipotent adipose-derived stem (hMADS) cells were transfected at confluence with miR-26a or miR-C (non-targeting control microRNA). Two days later (=d0), adipocyte differentiation was induced. At d9 after start of differentiation, cells were harvested to identify mRNAs that are down- and upregulated by miR-26a versus miR-C.

2013-11-25 | E-MARS-29 | biostudies-arrayexpress

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