Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of liver from flounder after injection with a vaccine containing killed Aeromonas salmonicida, the bacterium responsible for furunculosis

ABSTRACT: To study the effects of bacterial infection upon gene expression changes in flounder liver fish were artificially "infected" by injection with a commercial water-based vaccine containing killed Aeromonas salmonicida, the bacterium responsible for furunculosis, which, as its name describes, presents as external lesions (furuncles, "lumps"). This disease occurs in flounder as well as in salmonids. Flounders were treated by intraperitoneal injection with 1ml/kg Furunculosis vaccine (Schering-Plough, Aquavac Furovac 5, batch number FNM/C/007). 30 control fish were injected with 1% saline. Animals were then maintained unfed in static aerated sea water tanks in a constant temperature containment aquarium. Water was renewed every 2 days. After 1, 2, 4, 8, and 16 days, 6 vaccine treated and 6 saline control fish were removed, killed by a blow to the head and samples of liver tissue were immediately homogenized in TriReagent prior to gene expression profiling. Microarray experiments consisted an individual array for each of 4 or 5 fish from each timepoint and each treatment compared with an artificial reference sample.

ORGANISM(S): synthetic

SUBMITTER: Tim Williams

PROVIDER: E-MAXD-38 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:17alpha-ethinylestradiol (EE2) is one of the most potent estrogens that have the ability to interfere with the endocrine system of fish. The objective was to investigate the effects and mechanisms of action caused by 60 days of dietary exposure to 0.2 mg EE2/kg and 0.07 mg EE2/kg feed in female largemouth bass (LMB) during the reproductive season. Microarrays and pathway analyses were performed on hepatic tissues to identify genes and biological processes altered in female LMB by EE2 exposure. The hypothesis was that the two concentrations of EE2 would produce dose-response changes in sensitive genes. Body and ovary weights were measured and blood was collected for measurement of plasma steroid hormones (17beta-estradiol (E2), testosterone (T)) and vitellogenin (VTG) using ELISA. The 0.2 mg EE2/kg feed exposure reduced the gonadosomatic index (GSI) by 75%, and plasma levels of E2 and T were reduced by over 90%. Plasma VTG was increased by approximately 100% (from 4 to 8mg/ml) by the 0.2 mg/kg treatment. T levels, from the 0.07 mg EE2/kg feed, reduced GSI by approximately 30% and circulating E2 and T by ~80% but did not affect VTG concentrations. We found 1,594 and 1,165 genes were significantly affected (p<0.05) by the 0.07 mg EE2/kg feed and 0.2 mg EE2/kg feed, respectively. Gene ontology (GO) analysis revealed that there were different biological processes regulated by the two concentrations of EE2. Pathway analysis showed that the 0.07 mg EE2/kg feed exposure caused differential regulation of genes associated with fatty acid biosynthesis and glycolysis, indicating some metabolic effects. In contrast, the 0.2 mg EE2/kg feed exposure altered transcription of genes involved in immune response and apoptosis, suggesting a toxic response at this concentration. These results suggest that the two concentrations demonstrated distinct physiological responses, with the higher concentration inducing complete endocrine disruption in LMB. These findings demonstrate the usefulness of microarrays to identify possible biomarkers and modes of toxic action to dietary exposure in LMB. Two concentrations of EE2 would produce dose-response changes in sensitive genes. Female LMB were fed 5 days per week for 60 days with floating pellets that contained 0.07 or 0.2 mg/kg of EE2.

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Transcription profiling of liver from flounder after injection with a vaccine containing killed Aeromonas salmonicida, the bacterium responsible for furunculosis

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