Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of Streptomyces coelicolor wild type and phP-null mutants at different time points during phosphate starvation

ABSTRACT: S. coelicolor was grown in low-phosphate concentration R5 liquid medium which facilitated the transition to a phosphate starvation induced stationary phase and the subsequent induction of the PhoP regulon. Global gene expression profiling of a wild-type and a phoP-null mutant was performed using samples isolated, respectively, at 24, 30, 35 and 45 h of growth. RNA was extracted and the resulting cDNA was hybridised to S. coelicolor high density DNA microarrays.

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Emma Laing 

PROVIDER: E-MAXD-51 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Diverse control of metabolism and other cellular processes in Streptomyces coelicolor by the PhoP transcription factor: genome-wide identification of in vivo targets.

Allenby Nicholas E E NE   Laing Emma E   Bucca Giselda G   Kierzek Andrzej M AM   Smith Colin P CP  

Nucleic acids research 20120816 19

Streptomycetes sense and respond to the stress of phosphate starvation via the two-component PhoR-PhoP signal transduction system. To identify the in vivo targets of PhoP we have undertaken a chromatin-immunoprecipitation-on-microarray analysis of wild-type and phoP mutant cultures and, in parallel, have quantified their transcriptomes. Most (ca. 80%) of the previously in vitro characterized PhoP targets were identified in this study among several hundred other putative novel PhoP targets. In ad  ...[more]

Similar Datasets

Transcriptomics Transcription profiling of S. coelicolor grown in liquid culture and heat shock stimulated
2009-02-07 | E-MAXD-49 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Streptomyces coelicolor hspR mutant compared to wild-type strain
2009-02-07 | E-MAXD-44 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Streptomyces coelicolor wild type and zur deletion strains
2010-10-13 | E-MAXD-56 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Streptomyces coelicolor hspR+integrants of MT1110 after heat shock
2009-02-07 | E-MAXD-46 | biostudies-arrayexpress
Transcriptomics CGH of Streptomyces coelicolor versus S. lividans 66 and TK24
2011-10-01 | E-MAXD-58 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Streptomyces ceolicolor M600 and J2139 (sigR deletion) strains
2010-12-21 | E-MAXD-57 | biostudies-arrayexpress
Transcriptomics Chromatin immunoprecipitation of Streptomyces coelicolor wild type and phP-null mutants during phosphate starvation to determine binding locations of PhoP
2012-08-30 | E-MAXD-50 | biostudies-arrayexpress
Transcriptomics Comparative genomic hybridization by array of Ectocarpus siliculosus control reference reference hybridization
2010-04-27 | E-TABM-967 | biostudies-arrayexpress
Transcriptomics Differential expression of S. coelicolor M145 vs. tdd8 mutants in R5
2015-06-08 | E-MTAB-2644 | biostudies-arrayexpress
Transcriptomics Streptomyces coelicolor cells during various developmental stages: wildtype M145 vs. ΔSCO0204.
2016-04-14 | E-MTAB-4597 | biostudies-arrayexpress