Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

18 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human carcinoma LS174T cells following depletion of Myc and Usp28 using shRNA

ABSTRACT: Analysis of gene expression following depletion of Myc and Usp28 using shRNA in colon carcinoma LS174T cells.

INSTRUMENT(S): ScanArray Express HT [PerkinElmer]

ORGANISM(S): Homo sapiens

SUBMITTER: Nikita Popov

PROVIDER: E-MEXP-1070 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of mouse splenic B cells expressing a dominant-negative p300 allele which lacks histone acetyltransferase activity

Project description:The p300 protein is one of more than 15 known mammalian HATs. We have used mice that carry a mutant allele of the p300 gene, which encodes a full-length protein that, however, lacks detectable acetyltransferase (AT)-activity and that acts in a dominant-negative manner. In addition, the allele is conditional, so that it can be expressed in a tissue-specific manner with the help of mice that express Cre-recombinase. Expression of the mutant allele specifically in B-cells, using CD19-cre mice, results in premature death with full penetrance from an autoimmune disease that closely mimics systemic lupus erythematosus (SLE) in its pathological manifestations.

2006-07-01 | E-MEXP-747 | biostudies-arrayexpress

Transcription profiling of two mouse myc mutants Eilers-Mouse-MycKR6 vs Mycwt

Project description:Comparison gene regulation by two different Myc mutants

2005-10-17 | E-MEXP-407 | biostudies-arrayexpress

RNAi knock down of HectH9 in human osteosarcoma samples to identify myc regulated genes

Project description:Analysis of Gene Regulation by Myc in the absence of HectH9

2005-10-17 | E-MEXP-406 | biostudies-arrayexpress

Transcription profiling of blood vessel endothelial cells from wild type and PPARb knock-out mice

Project description:To elucidate the mechanisms underlying the perturbation of tumor vascularization in Pparb/ mice we performed matrigel plug assays23. Matrigel containing prostaglandin E2 (PGE2) and basic fibroblast growth factor (FGF-2) was injected subcutaneously into both wild-type and Pparb/ mice where it formed semi-solid plugs. These plugs became rapidly invaded by AQP-1 positive cells. To analyze differences between Pparb+/+ and Pparb/ samples we compare the gene expression profile of both samples by microarray analysis.

2007-12-01 | E-MEXP-983 | biostudies-arrayexpress

RNAi knock down of RET finger protein/ Tripartite protein in human osteosarcoma cell lines and analysis of Retinoblastoma protein dependent transcriptional changes

Project description:Analysis of Retinoblastoma protein (Rb) (Hs.408528) dependent transcriptional changes following siRNA mediated ablation of the RET finger protein/ Tripartite protein (RFP/TRIM27) (Hs.440382). Common reference design, two biological replicates with two technical replicates each.

2005-06-02 | E-MEXP-278 | biostudies-arrayexpress

Transcription profiling of whole lymph node RNA from mice with different Suv39h1 genotypes

Project description:Genome-wide expression analysis was performed on RNA from whole neoplastic lymph nodes derived from individual Eµ-myc transgenic and Eµ-myc transgenic; Suv39h1-/- mice and from normal wildtype spleens as control.

2008-02-20 | E-MEXP-1423 | biostudies-arrayexpress

Transcription profiling of short-term cultured Myc transgenic vs. Myc transgenic Suv39h1 deficient mouse lymphoma cells with and without exposure to 100 pM of human TGF-b1

Project description:Genome-wide expression analysis was performed on RNA from short-term cultured Eµ-myc transgenic vs. Eµ-myc transgenic; Suv39h1-/- lymphoma cells with and without exposure to 100 pM of human TGF-b1 for 24 hours, with wildtype B-cells as control

2008-02-20 | E-MEXP-1424 | biostudies-arrayexpress

Transcription profiling of human Soas-2 cells infected with adenoviruses expressing p53 proteins with charge-neutralizing or charge-inverting mutations in the H1 helix to investigate the role of the dimerization interface in p53 function

Project description:To investigate the role of the dimerization interface for p53 function, we introduced modest charge-neutralizing (R181→L "EL") and more severe charge-inverting (R181→E "EE" and E180→R, R181→E "RE") mutations into the H1 helix of the full-length p53 molecule. To characterize the nuclear transcriptional function of p53 mutants in an unbiased manner we employed gene expression profiling using cDNA microarrays. Saos-2 cells were infected with adenoviruses expressing the p53 proteins EE, EL, WT and RE which span the entire spectrum of apoptotic activity. Total cellular RNA was isolated 18 hours after infection when apoptosis had not yet occurred.

2010-04-01 | E-MEXP-1209 | biostudies-arrayexpress

siRNAi knockdown of ZBTB4 in human SH-EP cells reveals Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation

Project description:SH-EP cells were depleted for ZBTB4; Transfection of the cells were performed by oligofectamin RNAimax (Invitrogen) with siRNA smart pool (Dharmacon RNA Technologies); total RNA was isolated using RNAeasy Mini Kit (QIAGEN), preamplified, labeled with Cy3 and Cy5, respectively, and hybridized to cDNA-microarrays

2008-07-01 | E-MEXP-1554 | biostudies-arrayexpress

RNAi knock down of AURKA in human neuroblastoma IMR-32 cells

Project description:IMR-32 cells were infected either with control-shRNA or one of two different AURKA-shRNA vectors to identify genes regulated after AURKA knockdown.

2008-06-01 | E-MEXP-1496 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data