Transcription profiling of human A549 epithelial cells infected with polio virus or mock infected to compare Nanostring and Affymetrix technologies

ABSTRACT: Mock and poliovirus infected A549 cells were used as model system to assess Nanostring's technology for detecting mRNA transcripts, linearity of signal, reproducibility and fold change measurements. As a comparison, the same samples were measured using Affymetrix U133 2.0 plus arrays and several genes were followed up using RT-PCR. All samples for all platforms were performed in triplicate

ORGANISM(S): Homo sapiens

SUBMITTER: Gary Geiss 

PROVIDER: E-MEXP-1072 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress