Transcription profiling of wild type and mutant fission yeast exposed to different intensities of H202 and two other oxidants

ABSTRACT: We studied transcriptional responses of the fission yeast Schizosaccharomyces pombe to different intensities of H2O2 stress as well as two other oxidants: menadione sodium bisulfite and tert-butyl hydroperoxide. DNA microarrays were used to study the changes in expression profiles and the transcriptional regulation. We compare and contrast global expression and regulation of different intensities of H2O2 stress and different oxidants. The transcriptional response to low doses of H2O2 is very similar to menadione sodium bisulfite and the genes were controlled primarily by the transcription factor Pap1. The transcriptional response to medium and high doses of H2O2 is very similar to tert-butyl hydroperoxide and the genes were mostly regulated by the stress-activated MAP kinase Sty1p and the transcription factor Atf1p. We also compare global regulation of oxidative stress genes in fission and budding yeasts and discuss evolutionary implications.

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: dongrong chen 

PROVIDER: E-MEXP-1083 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress