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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of primary hippocampal neurons isolated from mouse E17 embryos and undergoing differentiation in the culture for 7h, 18h, 33h, 72h, 8 days, 12 days (at which time points the RNA was extracted) vs RNA isolated from a newborn brain

ABSTRACT: A time-course experiment, in which the gene expression in the primary hippocampal neurons isolated from E17 embryos and undergoing differentiation in the culture for 7h, 18h, 33h, 72h, 8 days, 12 days (at which time points the RNA was extracted) was compared by competitive hybridization with a common reference sample which was RNA isolated from a newborn brain.

ORGANISM(S): Mus musculus

SUBMITTER: Katleen Craessaerts

PROVIDER: E-MEXP-11 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene profiling of hippocampal neuronal culture.

Dabrowski Michal M Aerts Stein S Van Hummelen Paul P Craessaerts Katleen K De Moor Bart B Annaert Wim W Moreau Yves Y De Strooper Bart B

Journal of neurochemistry 20030601 5

We performed mRNA expression profiling of mouse primary hippocampal neurones undergoing differentiation in vitro. We show that 2314 genes significantly changed expression during neuronal differentiation. The temporal resolution of our experiment (six time points) permits us to distinguish between gene expression patterns characteristic for the axonal and for the dendritic stages of neurite outgrowth. Cluster analysis reveals that, in the process of in vitro neuronal differentiation, a high level ...[more]

PMID: 12753086

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