Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of microdissected human pancreatic tissues and pancreas cell lines (data in addition to E-MEXP-950)

ABSTRACT: Gene expression of pancreas ductal adenocarcinoma, normal duct cells, tumor stroma and stroma of chronic pancreatitis as well as of several cell lines. All primary tissue samples were microdissected. It is an addition of the data set E-MEXP-950.

ORGANISM(S): Homo sapiens

DISEASE(S): chronic pancreatitis

SUBMITTER: Christian Pilarsky

PROVIDER: E-MEXP-1121 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Activation of Wnt signalling in stroma from pancreatic cancer identified by gene expression profiling.

Pilarsky Christian C Ammerpohl Ole O Sipos Bence B Dahl Edgar E Hartmann Arndt A Wellmann Axel A Braunschweig Till T Löhr Matthias M Jesenofsky Ralf R Friess Helmut H Wente Moritz Nicolas MN Kristiansen Glen G Jahnke Beatrix B Denz Axel A Rückert Felix F Schackert Hans K HK Klöppel Günter G Kalthoff Holger H Saeger Hans Detlev HD Grützmann Robert R

Journal of cellular and molecular medicine 20080224 6B

Pancreatic ductal adenocarcinoma (PDAC) is characterized by an abundant desmoplastic stroma. Interactions between cancer and stromal cells play a critical role in tumour invasion, metastasis and chemoresistance. Therefore, we hypothesized that gene expression profile of the stromal components of pancreatic carcinoma is different from chronic pancreatitis and reflects the interaction with the tumour. We investigated the gene expression of eleven stromal tissues from PDAC, nine from chronic pancre ...[more]

PMID: 18298655

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Project description:Prostate cancer is characterized by heterogeneity in the clinical course that often does not to correlate with morphologic features of the tumor. Metastasis reflects the most adverse outcome of prostate cancer, and to date there are no reliable morphologic features or serum biomarkers that can reliably predict which patients are at higher risk of developing metastatic disease. Understanding the differences in the biology of metastatic and organ confined primary tumors is essential for developing new prognostic markers and therapeutic targets. Using Affymetrix oligonucleotide arrays, we analyzed gene expression profiles of 24 androgen-ablation resistant metastatic samples obtained from 4 patients and a previously published dataset of 64 primary prostate tumor samples. Differential gene expression was analyzed after removing potentially uninformative stromal genes, addressing the differences in cellular content between primary and metastatic tumors. The metastatic samples are highly heterogeneous in expression; however, differential expression analysis shows that 415 genes are upregulated and 364 genes are downregulated at least 2 fold in every patient with metastasis. The expression profile of metastatic samples reveals changes in expression of a unique set of genes representing both the androgen ablation related pathways and other metastasis related gene networks such as cell adhesion, bone remodeling and cell cycle. The differentially expressed genes include metabolic enzymes, transcription factors such as Forkhead Box M1 (FoxM1) and cell adhesion molecules such as Osteopontin (SPP1). We hypothesize that these genes have a role in the biology of metastatic disease and that they represent potential therapeutic targets for prostate cancer. Experiment Overall Design: Using Affymetrix oligonucleotide arrays, we analyzed gene expression profiles of 24 androgen-ablation resistant metastatic samples obtained from 4 patients and a previously published dataset of 64 primary prostate tumor samples. Differential gene expression was analyzed after removing potentially uninformative stromal genes, addressing the differences in cellular content between primary and metastatic tumors.

Dataset Information

Transcription profiling of microdissected human pancreatic tissues and pancreas cell lines (data in addition to E-MEXP-950)

Publications

Activation of Wnt signalling in stroma from pancreatic cancer identified by gene expression profiling.

Similar Datasets

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