MicroRNA Expression in HCC Tissues and Cells

ABSTRACT: Cell Lines, Cultures and HCC Tumor Tissues.
Malignant and non-malignant hepatocyte cell lines were obtained from the American Type Culture Collection (Manassas, VA) and cultured as recommended by the supplier. Total RNA from three paires of normal human liver tissue and from hepatocellular carcinoma was obtained from BioChain Institute, Inc. (Hayward, CA).

Isolation of MicroRNA.
Total RNA was obtained from cell lines and tissue samples using the Totally RNA isolation kit (Ambion, Austin, TX). The miRNA fraction was obtained by flashPAGE purification using the flashPAGEM-^Y Fractionator System (Ambion, Austin, TX). The size of the microRNA fractions were confirmed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Palo Alto, CA).

ORGANISM(S): Homo sapiens

DISEASE(S): normal

SUBMITTER: Fanyin Meng 

PROVIDER: E-MEXP-1125 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress