Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Comparative genomic hybridization of three strains of fission yeast grown with or without thiamine to repress the nmt1 promoter driving cdc18* expression and then harvested at three time points

ABSTRACT: JLP515, JLP1285, and JLP1288 were grown in EMM+LAUH supplemented with thiamine (B1) to repress nmt1 promoter driving cdc18* expression. Cells were grown at 32°C to late log phase, OD600 = ~0.500. Then cells were washed with water and used to inoculate a culture of EMM+LAUH lacking B1 and a culture of EMM+LAUH+ B1 to an OD600 = ~0.0125. Cells were put back at 32°C to grow. At 17, 21, and 25 hours post removal of B1, cells in EMM+LAUH-B1 were harvested for experimental samples. Cells in EMM+LAUH+B1 were harvested after 25 hours for control samples. Cultures were diluted periodically to ensure that cells remained in early log phase throughout time course.

INSTRUMENT(S): Axon GenePix 4000B scanning hardware

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Adam Rosebrock

PROVIDER: E-MEXP-1128 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparative genomic hybridization of three strains of fission yeast grown with or without thiamine to repress the nmt1 promoter driving cdc18* expression and then harvested at three time points

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