Transcription profiling of lung alveolar type II epithelial cells from CCSP-rtTA/(teto)7Stat3C bitransgenic mice treated with doxycycline and with spontaneous lung bronchoalveolar adenocarcinoma

ABSTRACT: Over-expression of Stat3C in lung alveolar type II epithelial cells of CCSP-rtTA/(teto)7Stat3C bitransgenic mice leads to severe pulmonary inflammation in the lung. As a consequence, spontaneous lung bronchoalveolar adenocarcinoma was observed in bitransgenic mice. Aberrantly expressed genes in the bitransgenic model were identified by Affymetrix GeneChip Microarray. Lungs were isolated from 4 individual mice in each group after 9 months of doxycycline-treatment. To eliminate sample differences generated by individual mice, the lung tissues were combined in each group and homogenized for RNA isolation. The total lung RNAs were purified using the QIAGEN total RNA purification kit as recommended by the manufacturer. The Affymetrix GeneChip assay was performed in duplicate. Briefly, the sa

ORGANISM(S): Mus musculus

SUBMITTER: Cong Yan 

PROVIDER: E-MEXP-1137 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress