Transcription profiling of pNCF (non-conjugative factor of F plasmid) carrying E.coli biofilm

ABSTRACT: Three E.coli affymetrix antisense arrays were used to examine the global gene expression of pNCF carrying E.coli biofilm. The pNCF is the derivative of only 65 kb non-conjugative factor of F plasmid. It was introduced to E.coli MG1655 strains before prepare the biofilm samples. The biofilms were cultured under the continuous flow cell system using MOP minimal medium supplemented with 0.02% glucose at 37C. The total RNA was extracted directly from the chamber after 48h of incubation for further array procedures according to the manufacture manual.

ORGANISM(S): Escherichia coli

SUBMITTER: Thithiwat May 

PROVIDER: E-MEXP-1145 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress