Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of pNCF (non-conjugative factor of F plasmid) carrying E.coli biofilm

ABSTRACT: Three E.coli affymetrix antisense arrays were used to examine the global gene expression of pNCF carrying E.coli biofilm. The pNCF is the derivative of only 65 kb non-conjugative factor of F plasmid. It was introduced to E.coli MG1655 strains before prepare the biofilm samples. The biofilms were cultured under the continuous flow cell system using MOP minimal medium supplemented with 0.02% glucose at 37C. The total RNA was extracted directly from the chamber after 48h of incubation for further array procedures according to the manufacture manual.

ORGANISM(S): Escherichia coli

SUBMITTER: Thithiwat May

PROVIDER: E-MEXP-1145 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Escherichia coli harboring a natural IncF conjugative F plasmid develops complex mature biofilms by stimulating synthesis of colanic acid and Curli.

May Thithiwat T Okabe Satoshi S

Journal of bacteriology 20080912 22

It has been shown that Escherichia coli harboring the derepressed IncFI and IncFII conjugative F plasmids form complex mature biofilms by using their F-pilus connections, whereas a plasmid-free strain forms only patchy biofilms. Therefore, in this study we investigated the contribution of a natural IncF conjugative F plasmid to the formation of E. coli biofilms. Unlike the presence of a derepressed F plasmid, the presence of a natural IncF F plasmid promoted biofilm formation by generating the c ...[more]

PMID: 18790864

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Dataset Information

Transcription profiling of pNCF (non-conjugative factor of F plasmid) carrying E.coli biofilm

Publications

Escherichia coli harboring a natural IncF conjugative F plasmid develops complex mature biofilms by stimulating synthesis of colanic acid and Curli.

Similar Datasets

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