Transcription profiling of mouse embryonic stem cells and primordial germ cells to identify genes up-regulated in pluripotent cells

ABSTRACT: Understanding the transcriptional regulation of pluripotent cells is of fundamental interest and will greatly inform efforts aimed at directing differentiation of embryonic stem (ES) cells or reprogramming somatic cells. We first analyzed the transcriptional profiles of mouse ES cells and primordial germ cell (PGCs) and identified genes up-regulated in pluripotent cells both in vitro and in vivo. These genes are enriched for roles in transcription, chromatin remodeling, cell cycle and DNA repair. We developed a novel computational algorithm, CompMoby, which combines analyses of sequences both aligned and non-aligned between different genomes with a probabilistic segmentation model to systematically predict short DNA motifs that regulate gene expression. CompMoby was used to identify conser

ORGANISM(S): Mus musculus

SUBMITTER: Miguel Ramalho-Santos 

PROVIDER: E-MEXP-1158 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress