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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of ERK1 and ERK2 knockdown in zebrafish embryos at blastula stage

ABSTRACT: ERK1 and ERK2 MAPKs are intensively studied signaling proteins that are involved in numerous cellular processes and signaling networks. However, their specific functions in embryonic development remain elusive. The upstream activation pathways for ERK1 and ERK2 are considered to be highly similar, and also many of their downstream targets are considered as common, but still relatively few in vivo downstream targets of these kinases have been identified conclusively. Recent mice studies indicate distinct roles for both ERKs in cellular proliferation, oncogenic transformation and development.

Microarray experiments were performed to compare morpholino mediated knockdown of ERK1 versus ERK2 in zebrafish development. These two conditions were compared to a standard control morpholino condition. To exclude possible effects of the control morpholino at a transcriptional level, we also performed the transciptome-analysis of the control morpholino, to an injection control (injection with phenol red).

INSTRUMENT(S): G2565BA DNA microarray scanner [Agilent]

ORGANISM(S): Danio rerio

SUBMITTER: S.F.Gabby Krens

PROVIDER: E-MEXP-1163 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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