Transcription profiling of wild type and sigma B mutant Liseteria monocytogenes to characterise the EGD-e sigma B regulon

ABSTRACT: Briefly, over night cultures of L. monocytogenes EGD-e cells (wild-type and sigma B mutant) were grown in brain heart infusion (BHI) broth (Difco). Bacteria were diluted 1:50 in 20 ml fresh BHI and incubated using a 100 ml Erlenmeyer flask at 37 °C with shaking (180 rpm, Unitron [Infors]). Bacterial cells were harvested at OD600 1.0 (3 h), OD600 2.0 (4 h), OD600 2.5 (8 h) OD600 2.5 (16 h) using RNAprotect (Qiagen) according to manufacturer.

INSTRUMENT(S): Generation III Array Scanner [Amersham]

ORGANISM(S): Listeria monocytogenes

SUBMITTER: Torsten Hain 

PROVIDER: E-MEXP-1170 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress