Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of heart from torpid and normothermic male Siberian hamsters compared at five different time-points

ABSTRACT: Heart cDNA from torpid and normothermic male hamsters is compared at five different time-points every three hours, starting from 2h prior to lights on and finishing 10h after lights on.

INSTRUMENT(S): GenePix 4000A [Axon Instruments]

ORGANISM(S): Phodopus sungorus

DISEASE(S): short day torpor

SUBMITTER: Fiona Crawford

PROVIDER: E-MEXP-1185 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Influence of torpor on cardiac expression of genes involved in the circadian clock and protein turnover in the Siberian hamster (Phodopus sungorus).

Crawford Fiona I J FI Hodgkinson Cassandra L CL Ivanova Elena E Logunova Larisa B LB Evans Gary J GJ Steinlechner Stephan S Loudon Andrew S I AS

Physiological genomics 20070911 3

The Siberian hamster exhibits the key winter adaptive strategy of daily torpor, during which metabolism and heart rate are slowed for a few hours and body temperature declines by up to 20 degrees C, allowing substantial energetic savings. Previous studies of hibernators in which temperature drops by >30 degrees C for many days to weeks have revealed decreased transcription and translation during hypometabolism and identified several key physiological pathways involved. Here we used a cDNA microa ...[more]

PMID: 17848604

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Project description:Plants are continuously exposed to a myriad of abiotic and biotic stresses. However, the molecular mechanisms by which these stress signals are perceived and transduced are poorly understood. To begin to identify primary stress signal transduction components we have focused on genes that respond rapidly (within 5 min) to stress signals. Because it has been hypothesized that detection of physical stress is a mechanism common to mounting a response against a broad range of environmental stresses, we have utilized mechanical wounding as the stress stimulus and performed whole genome microarray analysis of Arabidopsis thaliana leaf tissue. This led to the identification of a number of rapid wound responsive (RWR) genes. Comparison of RWR genes with published abiotic and biotic stress microarray datasets demonstrates a large overlap across a wide range of environmental stresses. Interestingly, RWR genes also exhibit a striking level and pattern of circadian regulation, with induced and repressed genes displaying antiphasic rhythms. Using bioinformatic analysis, we identified a novel motif overrepresented in the promoters of RWR genes, herein designated as the Rapid Stress Response Element (RSRE). We demonstrate in transgenic plants that multimerized RSREs are sufficient to confer a rapid response to both biotic and abiotic stresses in vivo, thereby establishing the functional involvement of this motif in primary transcriptional stress responses. Collectively, our data provide evidence for a novel cis-element that is distributed across the promoters of an array of diverse stress-responsive genes, poised to respond immediately and coordinately to stress signals. This structure suggests that plants may have a transcriptional network resembling the general stress signaling pathway in yeast and that the RSRE element may provide the key to this coordinate regulation. Experiment Overall Design: Three biological replicates of pooled plants were used for each treatment (Not wounded vs 5 min wounded). Each biological replicate is comprised of 2 technical replicates and a dye swap was performed for each technical replicate.

Dataset Information

Transcription profiling of heart from torpid and normothermic male Siberian hamsters compared at five different time-points

Publications

Influence of torpor on cardiac expression of genes involved in the circadian clock and protein turnover in the Siberian hamster (Phodopus sungorus).

Similar Datasets

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