Transcription profiling of yeast mutants Gal-YAH1, Gal-ATM1 and Gal-NBP35 to investigate responses to defects in cellular Fe-S protein maturation

ABSTRACT: The biogenesis of iron-sulfur proteins in eukaryotes is an essential process involving the mitochondrial iron-sulfur cluster (ISC) assembly and export machineries and the cytosolic Fe/S protein assembly (CIA) apparatus. To define the integration of Fe/S protein biogenesis into cellular homeostasis, we compared the global transcriptional responses to defects in the three biogenesis systems in S. cerevisiae using DNA microarrays. Microarray analyses were carried out with regulatable yeast mutants in which representatives of each of the three biosynthetic systems could be depleted. In particular, we used the mutants Gal-YAH1, Gal-ATM1 and Gal-NBP35.

INSTRUMENT(S): 418 [Affymetrix], ScanArray Express HT [PerkinElmer]

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ulrich Muehlenhoff 

PROVIDER: E-MEXP-1215 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress