Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human adipose derived stem cells before induction and at two time points after induction of differentiation

ABSTRACT: Adipose derived stem cells were induced for two time points (Day 2 and 28) and compared to the corresponding uninduced sample from the same human donor. A total of 20 biological replicates were conducted. Transcription profiling done with Affymetrix U133 Plus 2.0 arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: David Leong

PROVIDER: E-MEXP-1216 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

ATF5, a possible regulator of osteogenic differentiation in human adipose-derived stem cells.

Leong David Tai DT Abraham Mohan Chothirakottu MC Gupta Anurag A Lim Thiam-Chye TC Chew Fook Tim FT Hutmacher Dietmar Werner DW

Journal of cellular biochemistry 20120801 8

The regulatory pathways involved in maintaining the pluripotency of embryonic stem cells are partially known, whereas the regulatory pathways governing adult stem cells and their "stem-ness" are characterized to an even lesser extent. We, therefore, screened the transcriptome profiles of 20 osteogenically induced adult human adipose-derived stem cell (ADSC) populations and investigated for putative transcription factors that could regulate the osteogenic differentiation of these ADSC. We studied ...[more]

PMID: 22442021

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Project description:The cost of Carica papaya production through seed-based propagation is increased by sex segregation, making in vitro techniques a more appealing option for clonal propagation. Inducing embryogenic callus with 2,4-dichlorophenoxyacetic acid (2,4-D) hold the potential to large-scale cloning, although the molecular mechanisms underlying this process are still not well understood. In this study, we performed a temporal analysis in the proteome of C. papaya callus to identify the key players involved in embryogenic differentiation. Mature zygotic embryos were used as explants and treated with 20 μM 2,4-D to induce embryogenic callus. Total proteins were extracted at 0, 7, 14, and 21 days (T0, T7, T14, and T21), and 1407 proteins were identified using bottom-up proteomic approach. Comparative proteomics revealed 957 differentially accumulated proteins (DAPs) (p<0.05 and log2FC >0.585 or <-0.585) in at least one comparison between the analyzed induction times points. The clustering analysis revealed four clusters with distinct patterns of protein accumulation throughout the embryogenic callus induction treatment. The cluster 1 contains 386 DAPs that accumulated at all analyzed times after treatment with 2,4-D. In contrast, cluster 2 contains 165 DAPs that decrease in abundance during the induction. The cluster 3 contains 251 proteins that are most abundant just after the start of incubation in 2,4-D (T7) and cluster 4 grouped 155 proteins that accumulate after callus formation. Functional analysis revealed that proteins involved with reserve storage and seed maturation were more abundant in the explant at T0 and decreased as callus formation progressed. Biological processes involving carbohydrate and amino acid metabolism, aerobic respiration, and protein catabolic processes were enriched after induction treatment. Regulatory proteins, including histone deacetylase (HDT3) and argonaute 1, were more abundant after the start of induction treatment with 2,4-D, suggesting their role in acquisition of embryogenic competence. Predicted protein-protein networks revealed the regulatory role of proteins 14.3.3 accumulated during callus induction and the association of proteins involved in oxidative phosphorylation, hormone response, and SAM metabolism. Our findings emphasize the modulation of the proteome at different stages during embryogenic callus initiation and identify regulatory proteins that might be involved with the activation of this process.

Dataset Information

Transcription profiling of human adipose derived stem cells before induction and at two time points after induction of differentiation

Publications

ATF5, a possible regulator of osteogenic differentiation in human adipose-derived stem cells.

Similar Datasets

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