Transcription profiling of human umbilical vein endothelial cells after pulsed or continuous estradiol exposure

ABSTRACT: The experiment aims to investigate differences in the transcriptional response of human umbilical vein endothelial cells to estradiol treatment associated with the mode of administration. Human umbilical vein endothelial cells were treated for 5 days with a 1h pulse of 2.4 x 10-8 M 7β-estradiol (E2) once every day or with continuous 10-9M E2, ensuring cells received the same 24h E2 exposure (concentration x time) of two treatments. Control cells were treated with ethanol vehicle. For pulsed treatment, cells were washed and fresh medium was added after the 1h estradiol pulse. For continuous daily treatments, cells were incubated for 24h with media containing estradiol following the same scheme of media changes. The experiment was repeated at least three times.

ORGANISM(S): Homo sapiens

SUBMITTER: Farook Al-Azzawi 

PROVIDER: E-MEXP-1227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress