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Transcription profiling of human myeloma cell line IH-1 response to BMP-4

ABSTRACT: The IL-6-dependent human myeloma cell line IH-1 was stimulated with BMP-4 (50 ng/ml) for four hours and compared to control cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Torstein Ro

PROVIDER: E-MEXP-124 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of human post-mortem brain tissue (orbitofrontal cortex) from patients with bipolar disorder and matched healthy controls

Project description:Bipolar affective disorder is a severe psychiatric disorder with a strong genetic component but unknown pathophysiology. We used microarray technology (Affymetrix HG-U133A GeneChips) to determine the expression of approximately 22 000 mRNA transcripts in post-mortem brain tissue (orbitofrontal cortex) from patients with bipolar disorder and matched healthy controls. Orbitofrontal cortex tissue from a cohort of 30 subjects was investigated and the final analysis included 10 bipolar and 11 control subjects. Differences between disease and control groups were identified using a rigorous statistical analysis with correction for confounding variables and multiple testing.

2007-07-03 | E-GEOD-5389 | biostudies-arrayexpress

Transcription profiling of human brain tissue from subjects with bipolar disorder and healthy controls

Project description:Bipolar affective disorder is a severe psychiatric disorder with a strong genetic component but unknown pathophysiology. We used microarray technology (Affymetrix HG-U133A GeneChips) to determine the expression of approximately 22 000 mRNA transcripts in post-mortem brain tissue (dorsolateral prefrontal cortex) from patients with bipolar disorder and matched healthy controls. A cohort of 70 subjects was investigated and the final analysis included 30 bipolar and 31 control subjects. Differences between disease and control groups were identified using a rigorous statistical analysis with correction for confounding variables and multiple testing.

2007-06-24 | E-GEOD-5388 | biostudies-arrayexpress

Transcription profiling of human adult postmortem brain tissue from subjects with bipolar disorder and healthy controls

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2008-06-13 | E-GEOD-5392 | biostudies-arrayexpress

Transcription profiling of endothelial cells and dermal fibroblasts treated with anti-HCMV antibodies to induce modifications typical of scleroderma phenotype

Project description:Both human endothelial cells and dermal fibroblasts have been treated with anti-HCMV antibodies to induce modifications typical of scleroderma phenotype.

2005-08-01 | E-MEXP-382 | biostudies-arrayexpress

Transcription profiling of human adult postmortem brain tissue from Down syndrome and healthy control subjects

Project description:Down syndrome (DS) is the result of trisomy chromosome 21 but the mechanisms by which the genotype leads to the characteristic disease phenotype are unclear. We performed a microarray study using human adult brain tissue (dorsolateral prefrontal cortex) from DS subjects and healthy controls to characterise for the first time the human adult Down syndrome brain Experiment Overall Design: RNA extracted from human postmortem brain tissue from adult subjects with Down syndrome and healthy controls was hybridised to Affymetrix HG-U133A GeneChips to identify differentially expressed genes in the disease state.

2009-04-23 | E-GEOD-5390 | biostudies-arrayexpress

Transcription profiling of human HUVECs before and after Angiopoietin stimulation

Project description:Angiopoietin-Tie2 sytem has been inplicated in both vascular quiescence and angiogenesis. It is unclear how these two opposing signals are regulated from the same receptor-mediated intracellular signal transduction. We have noticed that Tie2 localization upon Angiopoietin stimulation depends upon the presence or absence of cell-cell contacts. Therefore, to identify the downstream signaling of Tie2 upon Angiopoietin stimulation, we performed DNA microarray analyais using RNAs obtained from confluent human umbirical vein endothelial cells (HUVECs) or sparse HUVECs stimulated with after Angiopoietin-1. There is striking difference on gene expression profile between confluent and sparse HUVECs. Experiment Overall Design: To identify the genes in HUVECs which are upregulated or downregulated by the stimulation with Angiopoietin-1 in the presence (confluent) or absence (sparse) of cell-cell contacts, mRNAs were prepared from either confluent HUVECs or sparse HUVECs stimulated or unstimulated with 100 ng/ml COMP-Ang1 (more potent Angiopoietin-1). RNAs were obtained from HUVECs three times cultured independently three times and mixed as one RNA sample for further DNA microarray analyses. Experiment Overall Design: Microarray (Human Genome U133 Plus 2.0 array, Affymetrix) analyses have been performed in duplicate. Biotin labeled probes were used for the series of analyses. Hybridization and Scan were performed according to the recommendation by Affymetric. Data have been processed following the MAS 5.0 with target intensity = 100.

2008-06-16 | E-GEOD-9677 | biostudies-arrayexpress

Transcription profiling of livers from female C57BL/6J and B6.C3H-6T mice to study differential gene expression in the two strains.

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2007-11-13 | E-GEOD-5959 | biostudies-arrayexpress

Transcription profiling of mouse lymphomagenesis induced by conditional expression of transgenic c-Myc oncogene in six different tumor cell lines

Project description:Lymphomagenesis was induced by conditional expression of transgenic c-Myc oncogene. Profile of genes expressed in tumor cells was compared with profile of genes in the same cell line treated for 12 hours with doxycycline to abrogate expression of transgenic c-Myc. Six different tumor cell lines were tested.

2004-09-17 | E-MEXP-169 | biostudies-arrayexpress

Transcription profiling of rat Perirenal and epididymal preadipocytes isolated from old and young individuals

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2008-06-15 | E-GEOD-6699 | biostudies-arrayexpress

Transcription profiling of S. cerevisiae response to nitrosative stress

Project description:Nitric oxide and NO-derived species (RNS) are defense molecules with broad antimicrobial activity. Micro-organisms have developed strategies to sense RNS and counteract their damaging effects. We used Saccharomyces cerevisiae, harbouring a deletion of YHB1 that encodes the main NO scavenger enzyme, to study consequences of RNS exposure on whole genome transcriptional response. The expression of >700 genes was altered on RNS treatment. No major role for ROS-scavenging enzymes was found, and the respiratory chain, the main site of ROS production, had only minor involvement in the RNS-induced stress. The changes were generally transient and also found after treatment with the respiratory inhibitor myxothiazol. 117 genes however showed a persistent response which was not observed after myxothiazol treatment. Of these, genes of the glutathione and DNA repair systems, iron homeostasis and transport were found up-regulated. Severe repression of genes of respiratory chain enzymes was observed. Many of these genes are known to be regulated by the transcription factor Hap1p suggesting that RNS might interfere with Hap1p activity. We showed also that Msn2/4p and Yap1p, key regulators of the response to, respectively, general stress and oxidative stress, played a role in mediating the RNS-induced response.

2006-03-02 | E-MEXP-580 | biostudies-arrayexpress

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