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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of wild type, DAL82 knock out, PDR3 knock out and UGA3 knock out yeast strains

ABSTRACT: Yeast knockout strains were constructed by the Yeast Deletion Project. Three biological replicates were analyzed for each strain. 5 ml cultures were inoculated at OD600 = 0.2 from saturated overnight cultures and grown to mid-log phase (OD600 = 0.6 - 0.8) in YPD media 37 or in phosphate-depleted media 38 at 30C. Cells were harvested by centrifugation and washed with nuclease-free water (Ambion). Total RNA was isolated immediately after harvest using the Ribopure Yeast RNA Isolation Kit (Ambion). 5 mg of total RNA was used to generate labeled probes with standard Affymetrix protocols.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ka Yee Yeung

PROVIDER: E-MEXP-1305 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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