Genotyping of humanglioblastoma tissue samples, cell lines and explants

ABSTRACT: glioblastoma multiforme genomic profiling by single nucleotide polymorphism microarray

Human GBM (glioblastoma multiforme)cell lines (U87, U118, U138, U343, U373, T98G) were maintained in Dulbecco's modified Eagle's medium with 10 % fetal calf serum, 10 U/ml penicillin-G, and 10 mg/ml streptomycin. All cells were incubated at 37 oC in 5% CO2.

Four primary GBM explants were established from patients with glioblastoma multiforme undergoing surgery as following described: Tumor specimens were immediately transported to the laboratory, finely minced to single cell suspension and cultured in complete medium [Ham's F-12/DME High Glucose medium containing 10% fetal calf serum, 10 U/ml penicillin-G, and 10 mg/ml streptomycin and 2 mM glutamax-1 into 100 cm2 tissue culture plastic dishes the second passage. All cells were incubated at 37 oC in 5% CO2.

GBM (glioblastoma multiforme) tissue samples were quick frozen.

Standard proteinase K-phenol-chloroform extraction method was used to extract DNA from GBM samples, cell lines and explants.

The matched peripheral blood data can be used as normalized data for their matched tumor tissue data.

The cell lines samples and two explants without normalized data, but they can be normalized by one of the peripheral blood DNA data.

ORGANISM(S): Homo sapiens

SUBMITTER: Dong Yin 

PROVIDER: E-MEXP-1330 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress