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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Trypanosoma cruzi transfected with TcPUF6, a pumilio like protein

ABSTRACT: Functional genomic characterization of mRNAs associated with TcPUF6, a pumilio-like protein from Trypanosoma cruzi

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Trypanosoma cruzi

SUBMITTER: Christian Probst

PROVIDER: E-MEXP-1355 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of differential gene expression during Metacyclogenesis in 2 developmental stages in T.cruzei

Project description:Evaluation of differential gene expression during the metacyclogenesis process in Trypanosoma cruzi, comparing three stages (epimastigotes, stressed epimastigotes and 24h adhered epimastigotes in differentiation) and using both total and polysomal mRNA.

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Transcription profiling of human NIH 3T3 inducible cell line to study the role of PKR in regulating gene expression

Project description:PKR is an interferon induced serine/threonine protein kinase, that is activated by double stranded RNA. PKR plays an important role in the antiviral defense by interferon. In addition to its role in translation, PKR participates in several signaling pathways to transcription. The goal of this experiment is to study the role of PKR in regulating gene expression in our NIH 3T3 inducible cell line, which could overexpress PKR wt protein after the removal of tetracycline (Donze O, Dostie J, Sonenberg N. (1999) Virology 256: 322-9).

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Transcription profiling of mouse tet inducible NIH 3T3 cells expressing ATPase-defective BRG-1 to identify SWI-SNF-Regulated Genes

Project description:NIH 3T3 cells (mouse fibroblasts) were engineered such that the dominant negative ATPase-defective BRG-1 is expressed via the tet-off inducible expression system to inactivate the SWI-SNF chromatin remodeling complexes (Mol Cell Biol 20, 2839). These cell lines have been used for several studies to demonstrate the in vivo functions of the SWI-SNF complexes in transcription, differentiation and cell cycle control. We used one of those cell line designated as B05-1. B05-1 cells were cultured in the medium with tetracycline for routine maintenance, and cultured in the medium without tetracycline for four days to maximally induce the expression of the dominant negative BRG-1, which we designated as B05-1(+tet) and B05-1(-tet) cells, respectively. The aim of our microarray experiments was to determine the genes whose expression levels are changed by inactivation of the SWI-SNF complexes by analyzing the genes differentially expressed between B05-1(+tet) and B05-1(-tet) cells. It should be noted that, since the comparison was made between B05-1 cells cultured with and without tetracycline for four days, the genes differentially expressed between these two cells do not only represent the genes that are directly regulated by the SWI-SNF complexes but also include the genes that are secondarily affected by SWI-SNF inactivation as well as the genes whose expression is changed by tetracycline effect per se.

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CTCF binding polarity determines chromatin looping

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Project description:Comparison of stomach and colon in normal mice.

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